She hit the headlines, crashed internet forums and caused a huge stir when she decided to flash her nipple live on stage.

But Madonna is not a woman who will bow down to her critics and if last night was anything to go by, she has only gone and fuelled the fire even further.

Because despite fans and commentators suggesting that now, at the age of 53, it’s time for her to cover up, Madonna then goes and shows off her bare bottom.

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Peachy or pathetic? Madonna whipped down her trousers and flashed her bottom in Rome on Tuesday night a few days after her nipple slip in Turkey

The veteran pop star took to the stage in Rome last night and instead of flashing her breast, this time she slipped off her trousers and showed off her bum to the audience.

During her song Human Nature, the tune during which she slipped her nipple out in Istanbul, she stripped off her microphone pack and unbuttoned her trousers, turned round, pulled them down and flashed her G-string clad derriere to the audience at the Stadio Olimpico.

Controversy: Madonna flashing her nipple on stage in Istanbul last weekend

However, it was a different story – and a different person – when she was snapped stepping out for lunch in Rome before the gig looking like the 53-year-old mother-of-four she is looking sophisticated in a blue dress and black peep-toe heels.

She was enjoying some down-time in the Italian capital ahead of the latest concert in her MDNA world tour.

She caused a stir in Istanbul when she launched into Human Nature and stripped down a black bra top, before pulling one of the cups down and exposing her breast.

Sophisticated: Madonna looked demure in a blue dress and peep-toe heels as she steps out in Rome

The singer received a backlash from fans and fellow celebrities for the over-exposure with Piers Morgan tweeting:’ Most embarrassing, cringe-worthy, desperate moment in the history of music?’

But some fans have been more sympathetic, like Hollywood actress Christina Applegate, who commented: ‘Reading twitter this am and there r all these tweets about Madonna and her nipple flash. And we are shocked because…?

‘What I’m saying is. Its not like Sarah Palin had a nip slip, its Madonna.’

Flashback: Madonna re-invented one of her iconic looks – the cone bra – for her new tour…

Other costumes on the extravagant tour included a red and white cheerleader outfit.

Following her Rome gig last night, she is set to perform in Milan and Florence later this week.

Give me a M, A…: Madonna also dressed as a cheerleader for another song